Determination of dissolved deoxyribonucleic acid concentration in lake water
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چکیده
منابع مشابه
Determination of dissolved deoxyribonucleic acid concentration in lake water
In this paper we d~scuss the sultablllty of the cetyltrimethylammonium bromlde (CTAB) precipitation method for the determination of dissolved DNA (dDNA) In lake water. Factors affecting dDNA recovery from the precipitate and further DAPI fluorometr~c assay were also examined. The proposed method was particularly suitable for routine determination of dDNA concentration in low and moderately eutr...
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A method for the quantitative determination of DNA in the 50 to 500 micromicrog. range is presented. Cells or cell nuclei are isolated individually from fixed tissue by means of micromanipulation. The tissue units in question are extracted in an oil chamber with deoxyribonuclease solution. The extracts are evaporated to dryness and redissolved to lens-shaped drops, the DNA contents of which are...
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Lakes in the boreal region have been recognized as the biogeochemical hotspots, yet many questions regarding the regulators of organic matter processing in these systems remain open. Molecular composition can be an important determinant of dissolved organic matter (DOM) fate in freshwater systems, but many aspects of this relationship remain unclear due to the complexity of DOM and its interact...
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The protein content and dry weight of tissue fragments may vary considerably without any real change in cell number. In view of the rather constant amount of deoxyribonucleic acid per cell found for any given species (1) it has been suggested that it should be used for the measurement of cell number (2). In tissue culture studies where it has been used in this way micro modifications of the pro...
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ژورنال
عنوان ژورنال: Aquatic Microbial Ecology
سال: 1996
ISSN: 0948-3055,1616-1564
DOI: 10.3354/ame011193